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camp dependent protein kinase inhibitor  (Tocris)


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    Tocris camp dependent protein kinase inhibitor
    Camp Dependent Protein Kinase Inhibitor, supplied by Tocris, used in various techniques. Bioz Stars score: 92/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/camp dependent protein kinase inhibitor/product/Tocris
    Average 92 stars, based on 17 article reviews
    camp dependent protein kinase inhibitor - by Bioz Stars, 2026-06
    92/100 stars

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    (A) Effect of PKA inhibitor on HSD3B2 expression in presence and absence of 8Br-cAMP stimulation was assessed in NCI-R cells grown either under normal (GM) or starved (SM) conditions for 48 h. Expression was assessed by Western blot analysis using specific antibodies. β-actin served as loading control. Representative Western blots are shown. Quantitative results summarize three independent experiments (mean±SD). (B) HSD3B2 activity under PKA inhibition in presence and absence of 8Br-cAMP stimulation in NCI-R cells grown either in normal (GM) or starved (SM) conditions for 48 h. HSD3B2 activity for converting [3H]DHEA to [3H]androstenedione (%) was assessed. Steroids were extracted and resolved by TLC as depicted. Quantification of three independent experiments (mean±SD) is shown. * p<0.05.

    Journal: PLoS ONE

    Article Title: Differential Regulation of Human 3β-Hydroxysteroid Dehydrogenase Type 2 for Steroid Hormone Biosynthesis by Starvation and Cyclic Amp Stimulation: Studies in the Human Adrenal NCI-H295R Cell Model

    doi: 10.1371/journal.pone.0068691

    Figure Lengend Snippet: (A) Effect of PKA inhibitor on HSD3B2 expression in presence and absence of 8Br-cAMP stimulation was assessed in NCI-R cells grown either under normal (GM) or starved (SM) conditions for 48 h. Expression was assessed by Western blot analysis using specific antibodies. β-actin served as loading control. Representative Western blots are shown. Quantitative results summarize three independent experiments (mean±SD). (B) HSD3B2 activity under PKA inhibition in presence and absence of 8Br-cAMP stimulation in NCI-R cells grown either in normal (GM) or starved (SM) conditions for 48 h. HSD3B2 activity for converting [3H]DHEA to [3H]androstenedione (%) was assessed. Steroids were extracted and resolved by TLC as depicted. Quantification of three independent experiments (mean±SD) is shown. * p<0.05.

    Article Snippet: Eight-bromoadenosine 3′,5′-cyclic monophosphate (8Br-cAMP), PD98059 mitogen-activated kinase kinase (MEK) inhibitor, U0126 MEK inhibitor and H89 cAMP dependent protein kinase (PKA) inhibitor were purchased from Sigma-Aldrich (Buchs, Switzerland) and dissolved in DMSO.

    Techniques: Expressing, Western Blot, Activity Assay, Inhibition